Introduction: Sepsis is one of the most common causes of morbidity and mortality in newborns, causing about 46% of global under-five child mortality. In sepsis, C - reactive protein (CRP) is elevated and Phosphate levels are low in blood and severity of hypophosphatemia also relates with poor outcome. The present study aims to measure levels of CRP, calcium (Ca), phosphate (P) and Ca:P ratio in cord blood of neonates with or without sepsis. Methods: Cord blood samples of 60 neonates were enrolled for the study. Samples were divided into two groups on the basis of presence or absence of sepsis in first 24 hrs of birth as decided by pediatrician. Measurement of CRP, calcium, phosphate, Calcium: phosphate ratio (Ca:P ratio) was done. Data so collected was analyzed with SPSS 20 software. Results: Neonates with sepsis had significantly high CRP and low phosphate levels in the cord blood. Ca:P ratio showed significant correlation with increasing CRP levels. Factors which did not show any significant correlation between the two groups were serum calcium levels, gestational age, birth weight and mode of delivery. 81.8% of neonates in group 2 (sepsis group) had phosphate level < 8.8 mg/dl(median level). Conclusion: Elevated CRP levels and Hypophosphatemia is present in neonates with sepsis. There is a negative correlation between serum phosphate and CRP levels. CRP has also been proposed as a diagnostic tool being easily measurable, affordable and convenient. Decreased Ca:P ratio should be taken care of while dealing with parenteral nutrition in such neonates. Phosphate levels must be carefully monitored in such cases.