This study assessed the effect of cabamate and pyrethroid pesticide residues from agricultural activities on fish samples from the Owena river, Ondo State, Nigeria. The aim of the study was to assess the bioaccumulation and the health risk implication of consuming polluted fish from the river. The fish samples were extracted with an ultrasonic bath extractor and the extracts were analysed with a gas chromatograph coupled to a mass spectrometry detector. The mean level of carbamate pesticide contaminants in dry season for Oreochromis niloticus, Gvmnarchus niloticus, S. galileaus and Claria gariepinus are 0.25±0.02µg/g, 0.24±0.01µg/g, 0.30±0.03µg/g and 0.26±0.02µg/g respectively. Their level in wet season for Oreochromis niloticus, Gvmnarchus niloticus, S. galileous, Clarias anguillaris and Parachanna obscura were 0.11±0.03µg/g, 0.21±0.02µg/g, 0.10±0.04µg/g, 0.14±0.03, 0.13±0.03µg/g and 0.12±0.05 µg/g respectively. The level of pyrethroid contaminants in dry season for Oreochromis niloticus, Gvmnarchus niloticus, S. galileaus and Claria gariepinus are 0.82±0.79µg/g, 0.53±0.04µg/g, 0.39±0.04µg/g and 1.91±1.99µg/g respectively. Their level in wet season for Oreochromis niloticus, Gvmnarchus niloticus, S. galileous, Claria gariepinus. Clarias anguillaris and Parachanna obscura were 0.22±0.05µg/g, 0.18±0.06µg/g, 0.13±0.05µg/g, 0.19±0.10, 0.12±0.02µg/g and 0.22±0.04 µg/g respectively. The concentrations of some of pollutants in the fish samples were higher than the FAO/WHO maximum residue limit of 0.5µg/g. The health risk evaluation indicates that there is no health risk. Nevertheless, strict monitoring of the handling and usage of these chemicals should continue to be enforced strictly by Nigerian government.