Obesity is a chronic condition characterized by excess body fat which results in an increase in the body weight of patients. C acutangula is a medicinal plant used in traditional medicine in Burkina Faso for the management of weight gain. The objective of this study was to determine the anti obesity potential of the bioactive fractions of C. acutangula extract. The ethanolic extract of C. acutangula was fractionated to obtain the hexanic, dichloromethane, ethyl acetate, butanol and aqueous fractions. The effect of these fractions on weight gain, food intake and lipid biochemical parameters were determined in vivo. The inhibitory activity of digestive enzymes (pancreatic lipase, trypsin) and the antioxidant potential were evaluated for these fractions. The butanolic fraction showed the best activity with an inhibition of 68.20 ± 3.4% at 100 μg / ml. The ethyl acetate fraction gave the best activity on reducing body weight with 18.11% reduction to 50mg / kg of body weight against the control (hyperlipid diet) which gave increases in weight of 34.83% respectively. The ethyl acetate fraction at 25 and 75 mg / kg of body weight and the butanolic fraction at 50 mg / kg showed the best reductions in the triglyceride level with respectively 111.89 ± 28.6; 108.35 ± 10.5 and 108.9 ± 10.21 mg / dl against the control which gave a rate of 280 ± 8.43 mg / dl. Best activities on cholesterol levels, HDLc and LDLc have been observed. For the antioxidant effect, the fractions caused a reduction in the level of MDA and an induction of the production of catalase. These results demonstrate that the ethyl acetate and butanolic fractions contain antiobesity molecules and could therefore be used in the implementation of a phytomedicine.