Introduction: Laboratory tests are important for the confirmation of the diagnosis of the disorders and also they are required to see the prognosis or to monitor the effects of the treatment of diseases. Many laboratory tests are ordered inappropriately, some of the ordered tests reports have not been utilized neither for the diagnosis nor for the assessing the prognosis of the disorders. Methodology: Source of data was obtained by documentation maintained in the inpatient registers for routine and emergency tests. Study type and design is Descriptive Cross Sectional study. Results: The percentage of total number of investigations increased was calculated from the year 2007 to till 2016. In our study we found that there was more than 1000% increase in the laboratory workload over a period of 10years both for elective and emergency (around 742%) sample analysis. Discussion: There is an increase in the workload of both routine and emergency tests which were done in Central Biochemistry laboratory and found out the normal value reports in some samples, which were required neither for the diagnosis nor for the prognostic purpose of the disorders. Conclusion: The study revealed gross overuse of laboratory; the ordered tests were required neither for the diagnosis nor to see the prognosis or to monitor the treatment of the disorders. This inturn affect the patients and organization in terms of direct cost & indirect cost. Inappropriate tests may increase the laboratory overload which affects laboratory results adversely in terms of the quality and availability of the laboratory resources.