Arabidopsisthaliana belongs to family (Brassicaceae) of mustard plant. It is widely studied because it has short generation time with life cycle of 6 week. There 20 cyclic nucleotide gated channel (CNGCs) genes in Arabidopsisthalianaout of which CNGC19 and CNGC20 are mainly involved in abiotic stresses like drought and salinity. The concerted effort is made to identify the role of CNGCs especially CNGC20 in salinity. Mutant lines of CNGC20 have been activated also identified potential role CNGC19 and CNGC20 in salt stress tolerance through activation tagging. It is functionally characterized CNGC20 for their potential role in salt stress. Knock out (KO) line was obtained from Salk Center and homozygous KO line was not sensitive to salt stress. Over-expression lines were developed and similar to KO line OX lines were also not tolerant to salt stress compared with wild type. Moreover, there was no significant difference between fresh weight of root and shoot.In addition, it has also been confirmed through public microarray databases that CNGC19 and CNGC20 are activated under salt stress in Arabidopsis. So it designed CRISPR/Cas construct for CNGC20 to develop double mutant of CNGC19 and CNGC20. The results indicate that KO and OX lines of CNGC20 are not significantly different than wild type under salinity, when compared with wild-type. However interaction of both CNGC19 and CNGC20 may result in clear information about their role in salt stress.