Plants species are most commonly used for metal phytoremediation in hydroponic as well as field experiments. The study remains unclear about methods measuring the growth parameters in Salix species. The aims of this review included the advanced methods that are used recently for measuring the growth parameters in Salix species. Inductively coupled plasma-optical emission spectroscopy is used to find out the concentration of plant phosphorus and cadmium. Nitrogen concentration detected by using the laboratory analyzer such as inductively coupled plasma-optical emission spectroscopy. Coomassie brilliant blue staining method is used commonly to determine the concentration of soluble proteins in plant samples. Biological activity of enzyme superoxide dismutase is measured by spectrophotometer at absorbance 560 nm. Lipid peroxidation in plant tissues determined by measuring the malondialdehyde formation using the thiobarbituric acid method. This method is based on spectrophotometric principle and red-violet complex is formed by the formation of malondialdehyde. Root activity in plant tissues determined by using the triphenyltetrazolium chloride test. The absorbance is then measured by spectrophotometer at absorbance 485 nm.