Infertility has grown into an emergent issue in recent times. It is defined as the inability of the couple to conceive despite two years of co habitation and unprotected exposure to pregnancy. As there is a dearth of studies concerning infertility among rural population this study was taken up in Sri Siddhartha Medical College, a constituent college of SSAHE, a Deemed to be university which caters to rural population of Tumkur district of Karnataka with the aim of assessing the role of HbA1c, Vitamin D and AMH in infertile women of the above mentioned population. Our case control study included age matched 40 infertile women and 40 apparently healthy women. Quantification of HbA1c was by fluorescence immunoassay on the Fine Care analyzer. AMH was assayed on cobas e immunoassay analyzer adopting chemiluminescene method. Vitamin D estimated by fully automated chemiluminescene analyzer Siemens Advia Centaur for 25-OH Vitamin D. Our study revealed statistically significant (P-<0.001) increase of Blood HbA1c and serum AMH whereas significant (P-<0.001) decrease in serum Vitamin D levels. The present study shows that there is high prevalence of vitamin D deficiency, significantly elevated HbA1c levels which is marker of long term glycemic control and increased AMH levels which indicated anovulation in infertile women. So it is suggested that early estimation of these biochemical parameters and their correction would possibly lead to increased chances of conception in these infertile women of rural population.