Postmenopausal women are at risk of cardiovascular disease due to the cessation of estrogen hormone secretion by the ovaries. Based on a few studies in the western population, the plasma homocysteine level has been shown to be a more reliable indicator of cardiovascular risk in elderly women, when compared to conventional markers like lipid profile. However, not many studies have been done in India regarding the role of homocysteine and lipid profile as a risk factor in causing the cardiovascular disease in postmenopausal women. Objectives: 1. To estimate plasma homocysteine and lipid profile levels in pre and post-menopausal women. 2. To compare and correlate homocysteine and lipid profile levels in pre and post-menopausal women. 48 confirmed cases of postmenopausal women and 48 regularly menstruating premenopausal women were included in the study after screening for inclusion and exclusion criteria. Blood samples were collected from the subjects and processed for estimation of lipid profile and plasma homocysteine levels. The BMI was significantly higher in the postmenopausal women as compared to premenopausal women. There was no significant difference in the mean values of lipid parameters between the two groups. The mean plasma homocysteine level (19.93 ±8.09) was significantly increased in postmenopausal women when compared to premenopausal women (10.52 ±2.62) and it also showed a positive correlation as age advances age. In our study we found that, there is a significant increase in homocysteine levels than lipid profile parameters, hence homocysteine may be consider as a sensitive marker of CVD’s in postmenopausal women.