Biotechnology is the emerging field of science that involved for the development of medical products medicines like vaccine based on DNA, recombinant vaccine genomics and proteomics. The purpose of this review included applications of biotechnology in healthcare. Several diseases remain undiagnosed at molecular level. By taken advantage through applications of biotechnology, many advancement has been taken in the in the field of medicine and health sciences. These medicines are alternative of traditional medicines which were used without any diagnosis. In PCR, amplification of gene product visualize in a specific manner. In gene therapy affected cells are replaced with normal cells it can be used for both somatic cells and germ cells. Fluorescence in situ hybridization has important applications to determine that how chromosomes arrangement involved in the behavior of an individual. Biotechnology also has other important applications in healthcare such protemics , genomics and gene therapy. Several vaccines have been made through using tools of biotechnology. There are some diseases that needed to treat at genetic level though healthcare biotechnology.