To prove the importance of DNA barcode one of the major point is that the genetic data is an important factor to improve the biodiversity conservation strategies and in fact at the different level of analysis in conservation biology many molecular tools. The aim of this review article is to access the role of DNA barcoding in biodiversity conservation in For the fine scale management of plant species, coral reefs, identification of cryptic and invasive species and regional management of fisheries in the field conservation biology molecular tools offer a great help. DNA barcoding had contribution in the conservation policy in such a way is that it came forward with the success of conservation action and other one is that it provide useful information about phylogenetic diversity and also about evolutionary history.