The effects of different concentrations of copper sulfate on the growth of and the accumulation of Cu2+ by root, shoot and leaf growth of sunflower (Helianthus annuus) were examined in this study. The purpose of our experiments was to consider sensitivity of the chosen 2 sunflower cultivars (Helianthus anus L.Cv.FH626 and FH674) to copper ions on the ground of physiological characteristics (content of leaf area, fresh matter, dry matter, amount of to show possible resistance mechanisms of this plant to copper ions. Afterwards they were poured with distilled water with the solution enriched with copper with concentration 20 mM CuSO4.5H2O and the last chosen combination was 40 mM CuSO4.5H2O. Waterings in the next phases of the experiment did not contain metal. In spite of the fact that no significant visual symptoms of toxic effect of metal were markedly noticeable, we detected decrease in the content of dry basis in the roots (less than 25–39% in comparison with the control of two tested cultivars treated by Cu cv. FH626 & FH674 tends to be the most resistant or tolerant to Cu toxicity from the point of view of evaluation of physiological parameters of particular cultivars.