Objectives: To compare the vitamin D levels between genders, age groups and geographical locations in a tertiary care hospital of Dakshina Kannada. Materials and Methods: A total of 208 (n=208) reports containing vitamin D levels were collected from Central laboratory, which were reported between January 2015 to December 2015. The values are tabulated in Microsoft excel sheet. Student‘t’ test was applied to compare the vitamin D levels between males and females. Whereas, ANOVA was applied for comparing vitamin D levels between different age groups and geographical locations. Results: Out of 208 vitamin D reports, 86 (41.3%) reports were from male and 122 (58.7%) from females. The mean values of vitamin D in males and females were 32.9±14.7 and 31.1±21.4 respectively. Even though females have low levels of vitamin D when compared to males but statistically it was not significant (p=0.512). The mean value of vitamin D from different geographical locations ranged from 20.9 ng/ml (from Ankola) to 78.6 ng/ml (from Dharwad) and 27.3±11.1 being noted from Dakshina Kannada. The intergroup comparison of vitamin D levels of individuals from different locations didn’t show any statistical significance (f =1.33 & p= 0.191).Similarly, there is no statistical significant difference in the vitamin D levels of different age groups. Conclusion: The present study concludes that there is no statistical significant difference in the vitamin D levels of males with females and also no significant difference was found in the vitamin D levels of different age groups and different geographical locations.