A study was carried out on forty four problem breeder cows to evaluate the therapeutic efficacy of GnRH and PGF2α for estrus induction response, time elapsed between treatment to estrus induction interval and conception rate and its effect on biochemical profile. Thirteen true anestrus cows were treated with GnRH (Inj. Receptal, 2.5 ml, IM, once), twenty three subestrus cows were treated with PGF2α (PGF2α, Inj. Estrumet, 2 ml, IM, once) and eight animals kept as untreated anestrus control. The estrus induction response, treatment to estrus induction interval, overall conception rate and service per conception in GnRH treated, PGF2α treated and positive control cows were 84.62%, 28.27±6.46 days, 72.73%, 3.13; 91.30%, 3.52±0.46 days, 71.43%, 2.53 and 50%, 48.25±10.8 days, 50%, 3.5 respectively. The plasma P4 level was significantly lower in all three groups as compared to other two periodic values (d-0 & d-20-22 post AI) which were due to luteal demise. The mean serum total protein was significantly (p<0.05) lower in untreated anestrus (8.57±0.36 g/dl) as compared to GnRH (9.75±0.28 g/dl) and PGF2α (9.62±0.31 g/dl) treated cows. However, Total cholesterol was numerically lowest in control cows than those of treated contemporaries but did not differ significantly (168.22±17.22; 208.46±14.71; 163.84±10.64 mg/dl; in GnRH, PGF2α and control group respectively, p<0.05). The Macro (Ca, P and Mg) and micro minerals (Zn, Fe, Cu, Co, and Mn) did not differ significantly among different groups. In conclusion, anestrus and subestrus cows can be well managed with GnRH and PGF2α therapy. The normal hormonal and biochemical milieu is essential for normal functioning of reproductive system.