In this Study, takes 219 milk samples (110 from cows and 109 from buffaloes) were screened for mastitis first with California mastitis Test (CMT) and risen cultural sensitivity test (CST) was performed on alt the positive samples from different villages of Banda Districts of Utter Pradesh in different seasons of the year. Data was analysed and overall animal season wise prevalence of mastitis was observed. Higher prevalence was observed in winter season in cows (27.52%) and in rainy season in buffaloes (33.94%) followed by winter season in cows and buffalos respectively. Antibiogram study was also done on all the samples and Enrofloxacin was found to be most sensitive antibiotic followed by Gentamicin and Ceftriaxone.