The type of fertilizer and fertilization techniques affect the biochemical of the rhizosphere. Different Nitrogen fertilizer inputs will have different effects in the process of releasing N ions, soil pH, number of colonies and respiration activity of soil microorganisms, so that it will affect the acquisition of Nitrogen (N) and Phosphorus (P). Meanwhile, different fertilization techniques will provide different stimulations to root growth, so that it will affect the acquisition of N and P, as well as the production of rattan seedling biomass. This study used rizoboxes which were designed as a 2-factor factorial experiment. Factor I: N-Urea ((NH2)2CO) and N-ZA ((NH4)2SO4), and factor II: Homogeny Fertilization and Localized Fertilization. The results showed that N-ZA input decreased soil pH, causing the highest number of microorganism colonies, but did not increase the respiration activity of microorganisms. N-Urea input also decreased soil pH and increased the number of microorganism colonies. However, both N-ZA and N-Urea showed no significant effects on N acquisition and production of rattan seedling biomass. Localized fertilization caused rooting to be concentrated in the fertilizing area, decreased N acquisition, otherwise increased P acquisition, but cannot increase rattan seedling biomass.