There are differences in the distributions of AB and Rh(D) blood groups amongst different populations. The present investigation was undertaken with the aim to study ABO blood group frequency amongst a blood donors in Sabratha city - Libya. The data for ABO blood group were collected from register of Central Blood Bank, Sabratha-Libya during a period of six months (January to June 2019). A total of 5356 donor were included in the study. The frequency of blood group O (43.19%) was found to be the highest, followed by blood group A (34%), B (18.13%) and AB (4.68%). The results also indicated that (83.53%) of donors were Rh positive and (16.47%) were Rh negative. The study may give preliminary idea about blood group frequency distribution among the population of Sabratha.