In this study, the bacteriological quality of bottled water in Mandalay, Myanmar was detected by the use of MPN method. Nineteen brands of Bottled Drinking Water (BDW) samples were analysed for total coliform count as the primary presumption of coliform contamination. 7 water samples were contaminated in a range from 6 MPN/ 100ml to 16 MPN/100ml. The faecal coliform, the indicator bacterium Escherichia coli was detected in 5 out of 19 samples by confirmation on EMB showing metallic sheen and completed tests were re-confirmed by further biochemical reactions. Antibiotic sensitivity and plasmid DNA extraction from the E.coli isolates were examined. (40%) of 2 out of 5 strains contained plasmids with molecular weights of more than 23kb. The overall results showed that 63.15 % among the subjected bottled water in Mandalay were noted to be microbiologically clean and safe for drinking.