Jatropha oil is considered one of the most important vegetable oil can use as biodiesel. This study was studied the genetic variations of Egyptian Jatropha curcas. Analysis of the five SSR loci with the ten of genotypes detected a total of 10 bands or alleles with 60 % polymorphism. The results showed that genotypes having the allele with the JCDS10 SSR locus (116bp) were 42 times more likely to have a high (above 10%) oleic acid content compared with these without this allele. The same results were obtained with the SSR loci JMDB57 and JCPS20 (alleles 82bp and 108bp) and increased odds to 3.8 and 3.6, respectively). While, allele at the JEM100 (140bp) SSR locus was showed negatively correlated with oleic acid content and exhibits a decreased odds (0.035) of having high (above 10%) oleic acid content. On other hand, Oleic acid content might be independent from the alleles at JCKASII SSR locus (OR=1.0). Moreover, the G9 oil specification was matched with European’s standard. The Values of Oil density, Viscosity and Iodine Value were 0.85 gm/cc, 34 cp and 96.5 mg. I2. g-1, respectively. Altogether, we can conculcated that the 5 SSR loci can use for study the genetic variations of Egyptian Jatropha curcas on a molecular basis. In addition, genotype 9 could be useful parental line for developing mapping population to map EST-SSRs (transcribed regions).