Spiders are one of the most diverse and ubiquitous group of animals. Despite being one of the most diverse groups of animals existing in India, their study has remained largely neglected. The present research paper provides a preliminary report on spider fauna of Ahmednagar city, M.S. India. Spider specimen were collected from different localities in and around the city during July 2015 to March 2016. Total 25 spider species representing 9 families and 19 genera were recorded. Results obtained shows that the Araneidae is the most represented family with 11 species followed by Salticidae, Oxyopidae and Lycosidae (03 species each) and 01 species each from Hersilidae, Pholcidae, Eresidae, Tetragnathidae and Thomisidae. Araneidae is the most dominant family (44% of species) followed by Salticidae (12% of species), Oxyopidae (12% of species) and Lycosidae (12% of species). A note on medical importance of spiders was also added. The outcome of the present research work will update data on faunal resources of Maharashtra and will be helpful for future researchers working on the spiders of the area. The findings will also be helpful for taxonomic and phylogenetic studies on spiders.