Sera from 67 bovines (cattle and buffaloes) from villages around Ludhiana city and 10 vaccinated animals from an organized dairy farm were subjected to Foot and Mouth Disease (FMD) DIVA ELISA. Sera from 10/67 field animals and 2/10 farm animals were found positive for the infection. Serial samples at 100 days, 130 days and 145 days postvaccination from 6 vaccinated buffaloes were assessed for titers of antibody against FMDV serotypes O, A and Asia-1 by Liquid Phase Blocking ELISA. The mean titers were as follows: Antibodies against O at 100 days, 130 days and 145 days = 2.4; Antibodies against A at 100 days = 1.858, at 130 days = 1.866, and at 145 days = 1.85; Antibodies against Asia-1 at 100 days = 2.225, at 130 days = 2.166, and at 145 days = 2.225, respectively. The differences among the mean titers of antibodies against FMDV O, A and Asia-1 at days 100, 130 and 145 were non – significant. However, two animals showed a titer of 1.65 against FMDV A at 100, 130 and 145 days which was below the protective level (> 1.8). The findings highlight the need for monitoring larger populations for the level of immunity induced by vaccination.