Photosynthesis or green synthesis of silver nanoparticles has been shown an ecofriendly and cost effective approach with a great significance of biomedical Applications. Plant extracts may act both as reducing agents and stabilizing agents in the nanoparticles formation. We therefore synthesized silver nanoparticles using Syzygium cumini methanol seed extract which is found to be most enriched in Phytonutrients, and had strong anti-glycoxidative potential. In this chapter, the Characterization of silver nanoparticles of S.cumini (ScSNPs) methanol seed extract was performed and their role on glucose stressed cardiac cells was studied. Nanoparticles synthesis is based upon its size, shape, disparity and surface area. After mixing with silver nitrate solution the colour changes from yellow to dark Brown confirming the formations of nanoparticles. This colour change is due to the Excitation of surface plasmon vibrations. We have synthesized the silver nanoparticles after 48 h of incubation but sometimes it takes more/less time depends upon the rateof bio-reduction of silver ion due to the presence of reactivity of enzymes and components exist in plant extract. The silver ions bind to the secondary metabolites inS. cumini and getting reduced. This complex of silver ion and metabolite interacts with other complexes and contributes to the formation of silver nanoparticle, which is a critical phenomenon and affected by pH, temperature, concentration and electrochemical gradient of silver ion. The plasmon resonance is acombined resonance of the conducting electrons of silver nanoparticles and scatteroptical light elastically with significant efficiency our study reveals that the polyphones and amines in S. cumini contribute to the formation of synthesis of silver nanoparticles and might go through a two-step redox process.