Our objective was to determine if polymorphisms associated with bovine Hsp90 gene have potential as a selection tool for productivity traits in cattle. Genomic DNA was extracted from buffy coat samples of crossbred Angus (n = 26) cows. Data were analyzed with cow as the experimental unit, genotype as the main effect, and dependent variables (calving rate, Julian calving date, calf birth weight, calf weight and cow weight at weaning, and calculated cow efficiency). When F-tests were significant (P < 0.05) least-squares means were separated using multiple T-tests. Polymorphism A97G is a transition from adenine to guanine at base 97 of the 283 base amplicon. Of the 26 cows, 19 were homozygous for adenine, 7 were heterozygous, and no homozygous guanine, which resulted in a minor allele frequency of 13.5%. Calving rate and cow weaning weight were not (P > 0.9) associated with genotype at A97G. However, 205-day adjusted calf weight was associated (P = 0.0002) with A97G genotype (188 vs. 208.1  7.1 kg; respectively AA and AG). That heavier calf weaning weight improved (P=0.08) cow efficiency for AG cows (Table 2). Heterozygous cows shed their winter hair coats earlier than AA cows. June hair coat score for AG cows (1.6  0.17) was lower (P < 0.03) than June hair coat scores for AA cows (2.2  0.11). Single nucleotide polymorphism A97G was associated with productivity traits in beef cattle. Our results suggest that additional research with this mutation is warranted to determine its value as a genetic tool for selecting animals that are less susceptible to heat stress and related problems.