The effect of extraction solvents (water, ethanol and hexane) on the content of phytochemicals such as alkaloids, saponins, total phenols, tannins and flavonoids in three selected medicinal ferns (Nephrolepis cordifolia (L.) Presl, Nephrolepis biserrata (Sw.) Schott and Pneumatopteris afra (Christ) Holttum) in Ekiti State, Nigeria was investigated. Matured leaflets were collected from healthy plants in Ekiti State University, Ado Ekiti, Nigeria. Extracts from shade dried and powdered leaflets of each of the ferns were subjected to phytochemical screening using standard methods. The results showed the presence of total phenols, tannins and flavonoids in all the extracts of the ferns while saponin was present only in water extracts. Hexane was not able to extract alkaloids and saponins in the three ferns. The results showed that extraction solvents significantly affected the phytochemical composition of the ferns. The water and ethanol extracts had higher content of the phytochemicals than the hexane extracts. The diversity of phytochemicals found present suggests that the ferns could serve as sources of useful drugs.