To study the antibacterial activity of Tabernaemontana divaricata (Apocynaceae) secondary metabolites capped silver nanoparticles (SNPs). In the present investigation, SNPs were synthesized using an aqueous extract of T. divaricata flowers. Flower aqueous extract was mixed with 1 m M silver nitrate for the biosynthesis of nanoparticles. The antibacterial activity of SNPs was determined against various bacterial cultures including laboratory isolates using the agar well diffusion method. The SNPs showed the highest antibacterial activity against Grampositive and Gram-negative bacteria. The present study envisions on the biosynthesis of SNPs from T. divaricata plant which are emerging as antibacterial therapy in modern medical applications.