The potential presence of naturally occurring antimicrobials in petals of flowers of Hibiscus sabdariffa L., (Malvaceae) was investigated against isolated eye pathogens. Owing to the usage of these flowers in common folklore medicine, the extracts of petals were screened for antibacterial activity against pathogenic microbes isolated from the eyes of eye infected persons. Bioactive compounds were extracted by cold extraction method, wherein Methanol, ethyl acetate and dichloromethane were used as solvents. The antibacterial activity of the extracts was assessed by agar well diffusion method. The study revealed that the extracts possessed antibacterial activity in a dose dependent manner. Among the tested flower extracts of Hibiscus sabdariffa, DCM extract showed better activity on most of the ocular pathogens tested. Hence the DCM extracts of petals of the flowers of Hibiscus sabdariffa can be used to discover antibacterial agent for developing new pharmaceuticals to treat eye infections.