Dengue and chikungunya are two mosquito-borne viral ailments transmitted by the same species of mosquito, Aedes aegypti and these ailments are of great public health concern in country like India. Thus, the present study was aimed to check the influence of antagonistic crustaceans on population of Aedes mosquitoes. Based on our laboratory experiments, the best ratio of antagonistic crustaceans (Cyclopoid copepod, Mesocyclops aspericornis and cladoceran, Daphnia magna) was chosen against Aedes aegypti larvae to test the influence of these crustaceans under simulated conditions. During the study it was observed that overall time taken for the larvae to convert into adult was 14.82±2.65 ranging from 14.0-21.0 days i.e. 4 days longer than the control sets. L1 larval stage was more prone to mortality i.e. 42.35±0.69 than other larval stages. Total adult emergence was significantly reduced i.e. 52.30±1.57 (31.65±0.69 males and 20.65±0.88 females). Significant reduction in body size of both male and female was observed i.e. 3.79±0.16 mm (control 4.44±0.07 mm) and female size significantly reduced to 4.17±0.15 mm when compared with control sets i.e. 5.48±0.08 mm. Life span of males was reduced from 15.35±1.20 to 9.33±0.33 days and in case of females longevity was reduced from 22.00±1.15 to 11.67±0.66 days.