A population of spiny-tailed lizard (Uromastyx aegyptia) exists in Hengam Island in the Persian Gulf. The field work was carried out over a period of 10 days by the use of a four-member group work in May 2015 through 10 random transects in the whole Island by the use of motorcycle. Once the animal’s cavity was found, some plots with dimensions of 10 by 10 meters were established over the center hole of the spiny-tailed lizard and habitat variables such as the region slope, geographical direction, type of the soil texture, percentage and number of plant cover according to species, distance to the nearest water source, road, and vegetation patches were measured. The habitat factors measured in the control points where there were no signs of the animal’s holes were also measured and compared with the present points. Ultimately, 28 holes of the spiny-tailed lizard in 7colony that had far from each other, were considered in the region. The results showed that the spiny-tailed lizard of the regions with a mean slope of 10% facing towards the north preferred rather semi hard soils (contain gravel and silt) (p<0.05). To protect the population of this species in the region, paying attention to some effective parameters such as the percentage cover of plant species, region slope and Acacia tree are of the most important factors for the use of the habitat of this species.