The aim of this study was to assess the self-perceived strategies on administration of antibiotic prophylaxis among dental practitioners. The study duration was of one month duration from June 2016 to July 2016. A self-designed structured and pretested questionnaire was administered among all the registered dental practitioners and dental institutional teaching faculty of Durg-Bhilai, Chhattisgarh. Questions regarding the common antibiotics prescribed and the indications for which the antibiotics prescribed were included in the questionnaire. Total of 127 dentists responded to the questionnaires with a response rate of 100%. It was observed that Amoxycillin (91.3%) was the commonly preferred antibiotics followed by fluoroquinolones (20.5%). Endodontic procedures (57.5%) and surgical procedures (45.7%) are most common procedures for which antibiotic prophylaxis were prescribed. The drug of first choice for patients with an allergy to penicillin was clindamycin (30.7%). 74.8% of respondents have never undergone any form of training on antibiotic prescription. Amoxicillin was the most commonly preferred antibiotics subsequent to different dental procedures. There is a clear need to emphasize correct diagnostic methods and develop contextualized prescription guidelines and educational initiatives, so that the optimum effect of antibiotics will be achieved without compromising patient‘s health.