The Murrel fish (Channa striata) is a of freshwater fish species, native to South Asian region and it contains high nutritional values. The murrel fish is a good source of Omega fatty acids and proteins, omega fatty acids and proteins helps to speed up the healing processes. For the present study Murrel Fish is purchased from local market of Aurangabad (MS) India. The fatty acid composition of oil extracted from Murrel fish has been investigated. Fatty acid composition of the oil is determined by Gas Chromatography. It is found that the Murrel Fish is rich in Palmitic acid, Stearic acid, Oleic acid, and Linoleic acid. Arachidonic acid, Behenic acid & Ricinoleic acid are also found in Murrel fish.