The adult earthworm Eudrilus eugenia are exposed to four sublethal concentrations such as 0.25gm/Kg, 0.5gm/Kg, 0.75gm/Kg and 1gm/Kg ZnO NPs. Biochemical and tissue damaging parameters are studied in Gut, Bodywall and Testis organs of both control and nanoparticles treated groups. The ANOVA results showed ZnO NPs treated groups results are significantly differed (p<0.05) than control group. Total Sugars, Total Cholesterol, Total Protein levels are significantly reduced whereas increased Urea, transaminase (GOT & GPT) and phosphatase (ALP & ACP) levels found in treated group organs. The results indicated that exposure of ZnO NPs through soil disturbed the basic biochemical compounds in tissues and their damages are indicated by increased enzyme levels.