This work aimed to study the effect of laser irradiation on bee honey (Seder) physical and chemical properties. Four honey samples were used in this study with 250 g of each; three of them were irradiated by He-Ne laser with output powers 1 mW, 1.5 mW and 2 mW for 5 minutes for each sample. Analysis included measuring of some honey physical and chemical properties for the irradiated and no irradiated samples particularly electrical conductivity, refractive index, density, viscosity, moisture, Ashes, wax, monocular sugars, total sugars, glucose, fructose, maltose, sucrose and acidity, it also included estimation of some elements like Na, Ca, K, Fe, Mg. The results show that irradiation by He-Ne lead to upward effect for some physical and chemical properties like electrical conductivity, monocular sugars and density, and it lead to downward effect for ashes, wax, sucrose and density, while it lead to no significant differences (P>0.05) on refractive index, viscosity.