Salmonellosis still remains as one of the major threat for poultry industry as well as public health in Bangladesh. Poultry is regarded as one of the most common reservoirs of Salmonella and contamination of poultry products can occur during any stage of poultry production. The current study was designed to identify Salmonella prevalence rate in broiler chickens and the antimicrobial susceptibility pattern of Salmonella spp. isolated from broiler chicken guts. A total of 320 bacterial colonies were isolated from broiler guts collected from 5 different markets of Sylhet city, Bangladesh, during January and February of the year 2017. The samples were subjected for isolation and identification of Salmonella spp.and through a series of morphological and biochemical observations, 14.37% of the isolates were identified as Salmonella. Antimicrobial sensitivity test was done against 8 commonly found antibiotics in chicken carcasses. Salmonella strains were found to be completely resistant against Ampicillin (100%). The frequency of antibiotic resistance ranked in the following order: Erythromycin (97.82%), Tetracycline (84.78%), Polymyxin B (73.91%), Co-trimoxazole (58.7%), Neomycin (13.04%) and Ciprofloxacin (6.52%). However, Salmonella isolates showed considerably greater sensitivity against Gentamycin (95.64%) and Ciprofloxacin (80.43%). Multidrug resistance was found to be present in all the isolates which is alarming for poultry business and consumer as well.