The present study was aimed to develop the high performance thin layer chromatography (HPTLC) fingerprint profile for methanol extracts of marine red alga Spyridia. fusiformis. A HPTLC method for the separation of the active constituents in extracts have been developed and TLC of these extracts on silica gel pre-coated aluminum plates by the automatic TLC applicator and using the solvent system n-hexane: ethyl acetate (60:40 v/v) was performed. Preliminary phytochemical screening of the extracts showed the presence of alkaloids, triterpenes, tannins, saponins, glycosides, phenolic compounds and flavonoids. HPTLC finger print scanned at 350 nm from methanol extractof S. fusiformis revealed 7 peaks with Rf values in the range of 0.32 to 0.92. It can be concluded that HPTLC fingerprint analysis of methanol extract of marine red alga S.fusiformis a phytochemical marker.