Microorganisms are important sources of bioactive natural products with enormous potential for the discovery of new molecules for drug discovery, industrial use and agricultural applications. In this study, photochemical analysis of the extracts of secondary metabolites produced by endophytic fungi from leaves of Irvingia gabonensis was carried out using recommended standards. Results revealed the presence of alkaloids, flavonoids, saponins, tannins, steroids and glycosides. Extracts from all five cultures of the endophytic fungi gave a wide variety of phytochemicals, except saponin. From the results, the different natural products fractions are a potential in the provision of the basis for the synthesis of novel therapeutics that would aid in the fighting of life threatening diseases once resistance builds up. Therefore, the search for the secondary metabolites that could enable the synthesis of drugs should be encouraged. The potential value of investigating metabolite production by endophytic fungi from leaves of Irvingia gabonesis have been demonstrated from the result to be a source promising antimicrobial compounds against some human pathogenic microbes.