The aim of this study was to assess and compare blood glucose, lactate and pyruvate concentrations changes during Wingate exercise and 10 min post-exercise recovery in football players with different aerobic capacity levels. Blood glucose, lactate and pyruvate concentrations were measured in 27 amateur football players during supramaximal Wingate anaerobic test (WAnT). The subjects were divided into two groups: group1, 16 subjects with maximum oxygen uptake (VO2max) values whose were higher than 60 ml/kg/min; group 2, 11 subjects with VO2max values lower than 55 ml/kg/min. All subjects performed WAnT after determining the VO2max using an incremental test. The WAnT was performed on an Ergomedic cycle ergometer. Blood samples were collected at rest, at the stop of WAnT and during the 10 minutes’ recovery (following WAnT). Only slight and no significant decrease was observed during WAnT for blood glucose: 5.5±0.3 mmol/L vs 4.9±0.3 mmol/L, group 2. Very large increases in lactate and pyruvates concentrations were found at the WAnT stop: 12.41±0.15 mmol/L vs 1.57±0.14 mmol/L for group 1, 13.87±0.12 mmol/L vs 1.84±0.25 mmol/L for group 2. The peaks of [La] were observed at 2 min post-exercise WAnT: 12.83±0.23 mmol/L and 14.59±0.32 mmol/L respectively. The same trend was observed for pyruvate concentrations. Blood [La] concentrations measured during 2 min to 10 min recovery decreased significantly, and were significantly and negatively correlated with VO2maw. This study clearly confirm that a brief and supramaximal Wingate exercise induced higher lactate and pyruvates concentrations increase in football players with high VO2max level than others with low VO2max level. The major responsible factor is glycolytic capacity.