Abstract: The prime aim of the investigation was to compare Mental Toughness of Physical Education Teacher Trainees and Assam Police Trainees (AP). To obtain the data researchers selected total sixty four (64) subjects ,thirty two (n1=32) male Physical education Teacher trainees from Centre for Studies in Physical Education and sports, Dibrugarh University and thirty two (n2=32) male Assam Police trainees from 2nd Assam Police Battalion Camp, Makum, Tinsukia, Assam, India between the 19-22 years age. The study was delimited to assessment of Mental Toughness by using questionnaire mental toughness by Dr. Alan Goldberg (1998). The Independent t-test was applied to assess the differences between the groups. The level of significance was set at 0.05 of confidence. Results revealed statistically significant differences between the two groups of overall mental toughness (t0.05 (62) = 7.55 > 1.67) and also significant difference was found sub-variable i.e. Motivation (6.26>1.67), ability to handle the pressure (33>1.67), concentration (8.44>1.67), confidence (8.05>1.67) and Rebound ability (3.28>1.67).