With the development of globalization, cross-cultural communication has become more and more important. Under different cultural backgrounds, interpersonal relationships vary greatly. The differences in interpersonal relationship have an important influence on the communication between Chinese and American people. Exploring the differences of interpersonal relationship between Chinese and Americans is of great significance for guiding people to better cross-cultural communication. By the comparative analysis of the interpersonal relationship between Chinese and Americans from three aspects: family relationship, friendship and teacher-student relationship, this paper explores the specific manifestation of differences and the causes behind them, and further puts forward reasonable solutions to the promotion of the cross-cultural communication between the two peoples.