Prussian blue has crossed a variety of disciplines. Prussian blue was found by chance in Berlin at the beginning of the eighteenth century, and was then the first synthetic dye used on a large scale, leading to a revolution in artistic expression throughout Europe and Asia. It was deep, stable in color, which changed the way paintings, printmaking, and textiles were made, and in time influenced some of the greatest artworks of all time. The pigment was used in medicine as well as art. Max Perls, in 1867, modified the chemistry of Prussian blue to make it suitable as a stain for tissues for ferric iron, which is one of the longest-lasting stains used in diagnostic pathology. Perl's' Prussian blue stain is an essential part of the evaluation of iron overload disorders, pulmonary hemosiderosis, myelodysplastic syndromes, and forensic work today. Incredibly, Prussian blue is also an FDA approved therapeutic agent for radioactive cesium and thallium poisoning. Prussian blue's development from an accidental laboratory contaminant, a scientific curiosity, and an antidote is a testament to how one molecule can have a lasting impact on human knowledge through serendipity, curiosity, and interdisciplinary innovations. The review overviews the history and chemical basis, pathological uses, forensic importance, therapeutic uses, and future potential of Prussian blue.