Diabetic foot is a severe complication of diabetes, the management of which depends on the microbiological profile and antibiotic resistance patterns. This study aimed to describe the bacteriological profile and antibiotic susceptibility of the isolated bacteria. A retrospective study conducted from 2019 to 2025 at the Mohamed V Military Training Hospital in Rabat included 100 patients hospitalised for diabetic foot infection. Clinical and bacteriological data, along with antibiograms, were analysed. The study population was predominantly male (80%; M/F sex ratio = 4), with a mean age of 68.2 years. Among the 125 isolates identified, Gram-negative bacilli predominated (50.4%), followed by Gram-positive cocci (46.4%). The main species isolated were Staphylococcus aureus (18.4%), Klebsiella pneumoniae (13.6%), Proteus mirabilis (11.2%), Enterococcus faecalis (9.6%) and Pseudomonas aeruginosa (8.8%). Among the Staphylococcus aureus strains, 17.4% were methicillin-resistant (MRSA). Among Enterobacteriaceae, 16.0% were producers of extended-spectrum beta-lactamases (ESBL), and a single isolate was a carbapenemase producer. Multidrug-resistant strains of Pseudomonas aeruginosa were observed in 27.3% of cases. By documenting the local bacterial flora and resistance burden, this study highlights the high prevalence of multidrug-resistant pathogens in diabetic foot infections, demonstrating the essential need to adapt empirical antibiotic regimens to regional epidemiology and systematically guide targeted therapy using antibiogram results. Keywords: Antibiogram, antibiotic therapy, bacterial resistance, bacteriological profile, diabetic foot, extended-spectrum beta-lactamases, infection, Staphylococcus aureus.