A fish may be defined as a poikilothermic, aquatic chordate whose appendages, when present, are developed as fins, whose chief respiratory organs are gills and whose body is usually covered with scales. The present study was undertaken to document the fish diversity of River Panjkora in District Dir Lower, Khyber Pakhtunkhwa, Pakistan. Samples were collected from March to August 2024 using gill nets, cast nets, hooks and automatic rods at five sites along the river, namely Talash, Shagokas, Balambat, Rani and Khall. A total of 81 specimens were captured and identified, representing six species distributed across two orders (Cypriniformes and Perciformes) and two families (Cyprinidae and Channidae). Cyprinidae was the richest family, comprising five species Schizothorax esocinus, Puntius ticto, Tor macrolepis, Carassius auratus and Crossocheilus diplocheilus whereas Channidae was represented by a single species, Channa gachua. Crossocheilus diplocheilus was the most abundant taxon (35 individuals; 43.20%), while Carassius auratus was the least abundant (3 individuals; 3.70%). Morphometric measurements and fin formulae were recorded for every species; the smallest species was Puntius ticto (total length 8.2 cm) and the largest was Tor macrolepis (total length 19.5 cm). Simpson's index of dominance was 0.255 and the reciprocal Simpson's index was 3.92, indicating a moderately diverse assemblage dominated by cyprinids. Fishes were identified using standard taxonomic keys, including Fishes of the Punjab (Mirza and Sandhu, 2007) and the Urdu key of Mirza (1990). The findings indicate that the river can potentially harbour additional species and could serve as a resource for conserving threatened taxa. Regular monitoring of the fish fauna, periodic assessment of water quality, stocking of native fish and control of illegal fishing are recommended to safeguard the ichthyofauna.