Hepatitis C virus (HCV) is a single-stranded, blood-borne, RNA virus, which infects hepatocytes and can cause chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. The task of this hospital-based study was to identify the level of HCV infection in patients in some of the districts of Khyber Pakhtunkhwa, Pakistan. There were 250 patients attending Royal Imperial Hospital, Mingora, to have pre-surgery assessment. Data analyses were performed based on gender, age group and district of residence. Blood samples were taken, serum/plasma was separated and HCV screening was done by the use of rapid diagnostic device. Among 250 patients, 7 were positive of HCV which constitutes a total of 2.8 prevalence. The positive rate of males was 4 out of 104 with a prevalence of 3.84, and the positive rate of females was 3 out of 146 with a prevalence of 2.05. Analysis by district indicated that most positive cases were in Swat with Shangla having the least prevalence. The distribution in terms of age showed that HCV positivity was more common among the adult age groups than among the young people. The results indicate that HCV is a health issue to the community of the study area especially adults and males. To decrease transmission, better screening, awareness, safe blood transfusion practices, sterilization of surgical and dental instruments, and avoidance of sharing needles are advised.