The study was carried out to detection of H.pylori in [200] patients who attended two teaching hospitals in Baghdad. The diagnosis done by Immunochromatography methods. Stools and blood Samples was taken from each patient as well as other [30] healthy control matching in their age. The study included detection the Levels of Interleukin-27 and Matrix Metalloproteinase-9in sera of the patients and the control. The result indicated presence of H pylori antigen in 115 cases 69 cases of males and 46 of females, Also, the results indicated increasing levels of Interleukin-27 and Matrix Metalloproteinase-9 in patients’ sera in comparison with healthy control.