Urinary tract infections [UTIs] are more frequently linked to Proteus mirabilis. To isolate P. mirabilis, 100 urine samples were taken from patients at the City of Medicine Teaching Hospital in Baghdad City who had recurrent UTIs. and look into the ureC gene's distribution. The purpose of the current study was to examine the ureC genes in Gram-negative bacterial isolates from UTI patients and how they differed among bladder cancer patients. Patients with urinary tract infections [UTIs] provided one hundred urine samples. Fifty urine samples from patients with bladder cancer were included in the first group, while fifty urine samples from patients without the disease were included in the second group. The age range of the groups was 15 to more than 65. Using biochemical testing, the gram-negative bacterial species responsible for UTIs were examined and identified. Gender, age groups, and bladder cancer were among the variables linked to UTIs that were investigated. The P. mirabilis isolates were identified based on the morphological and biochemical features of the cultures, and the diagnosis was confirmed using the VITEK 2 compact system. The polymerase chain reaction [PCR] method using ureC genes was used for final identification. The distribution of P. mirabilis by age was significant, with a higher distribution in the 50–55 age range. IL-6: control 13.2+2.8 pg/ml, patients with urinary tract infections [UTIs] and with bladder cancer 12+1.9, patients with urinary tract infections [UTIs] with non-bladder cancer 13.5+4.1. IL-8 levels were: control 1542+123.2 pg/ml, patients with urinary tract infections [UTIs] and with bladder cancer 2542.8+265.8, patients with urinary tract infections [UTIs] with non-bladder cancer 3212+231.2