Background: Health care waste management constitutes one of major public health concerns of modern health care. Since the healthcare workers are at the forefront of the waste generation, their knowledge on its management and hazard prevention is a major determinant of safe handling practices and effective infection control. Methodology: Present study has been conducted as a cross-sectional study among a convenient sample of health care workers, in King Salman Hospital Riyadh (N=131), during July-August 2024. A self-administered questionnaire was used to collect the quantitative data, in both English and Arabic language. The questions were asked under three main domains of the waste management; segregation, transportation and hazard prevention. Result: Out of total 133 study participants, majority of our study participants were nursing staff (77%, 103/133), in the age group of 31-40 years (54%, 72/133) and been working at our hospital for 2-5 years (46%, 61/133). For the survey items, the positive responses ranged from 72%-98%, 81-96% and 81-100% among the segregation, transportation and hazard prevention domains respectively. Conclusion: We have observed a high level of knowledge and awareness regarding hazardous waste management among healthcare workers, reflecting the efforts taken for continuous capacity building.