Green synthesis of silver nanoparticles (AgNPs) has emerged as a sustainable, cost-effective alternative to traditional chemical and physical methods (Sati et al.,2025). This study explores the plant-mediated synthesis of AgNPs using bioactive extracts, which serve as both reducing and stabilizing agents. The resulting nanoparticles exhibit unique tunable optical properties, primarily characterized by strong Surface Plasmon Resonance (SPR). We demonstrate that these biogenic AgNPs significantly enhance the detection sensitivity of SPR-based biosensors compared to conventional substrates. Characterization through UV-Vi’s spectroscopy, SEM, and EDX confirms the formation of spherical AgNPs with high elemental purity. These findings provide a roadmap for the development of eco-friendly, high-performance diagnostic tools for environmental and biomedical monitoring.