Objectives: This study aimed to investigate the effect of fartlek training combined with different running based high intensity interval training on cardio respiratory endurance of men soccer players. Methods: The experimental study enrolled 60 men soccer players who taking part in the inter collegiate level football tournament from University, Calicut Kerala. The age ranged between eighteen to twenty-one years, which were selected to carry out the research. The subjects who met the inclusion criteria were selected using random sampling and divided into three intervention groups and a control group. The test items used to collect the cardio respiratory endurance was tested by coppers 12 minutes run or walk test and performance recorded in meters. The 12 weeks of stipulated training was provided to the participants for the period of 60-minute sessions for the three alternative days in a week. Statistical Procedure: To process the results of the study, analysis of the data was done with the IBM SPSS Statistics 22 software. The statistics obtained were provided by way analysis of co variance. The level of confidence was fixed at 95% and values below p<0.05 were considered significant. Results: The one-way analysis of co variance showed a significant (p<0.05) improvement in cardio respiratory endurance over the 12 weeks of stipulated training. Conclusion: The results indicate considerable significant improvement on cardio respiratory endurance was observed. The findings indicate that the selected training regimes led to significant improvement on cardio respiratory endurance.