Background: Cucumis sativus (cucumber) is a widely consumed fruit known for its high water content and antioxidant properties. Despite its numerous health benefits, limited research has been conducted on its potential effects on male reproductive health. This study investigated the impact of cucumber fruit juice on male reproductive hormones and semen quality parameters. Methods: Male Wistar rats were divided into three groups: a control group and two experimental groups receiving 10 ml/kg and 20 ml/kg of Cucumis sativus fruit juice daily for four weeks. Serum samples were analyzed for follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone levels using enzyme-linked immunosorbent assay (ELISA). Semen samples were evaluated for sperm motility, viability, morphology, and abnormal sperm cell count. Results: The administration of cucumber juice did not significantly affect FSH, LH, or testosterone levels (p > 0.05), indicating that the fruit juice does not interfere with endocrine regulation of male reproduction. However, sperm quality parameters showed significant improvements. Sperm motility increased notably in the experimental groups, with the highest motility observed in the 20 ml/kg group. Sperm viability and normal morphology were also significantly enhanced compared to the control (p < 0.05). The percentage of sperm abnormalities, including head defects, mid-piece distortions, and cytoplasmic droplets, was significantly reduced, particularly at the higher dose. These findings suggest that cucumber juice may improve sperm function without altering hormonal balance. Conclusion: The results indicate that Cucumis sativus fruit juice has a beneficial effect on sperm quality, enhancing motility, viability, and morphology while reducing sperm abnormalities. These improvements may be attributed to the antioxidant and hydrating properties of cucumber, which help to reduce oxidative stress and support testicular function. However, the absence of significant hormonal changes suggests that its effects on male fertility are primarily local rather than systemic.