Background: Gingival biotype (now often termed periodontal phenotype) describes gingival thickness, keratinized tissue width, and underlying bone morphology. This phenotype has significant influence on periodontal disease progression, clinical outcomes, aesthetic stability, and response to therapy. Aim: To assess gingival biotype distribution in a defined population and evaluate its association with periodontal parameters, specifically probing depth and gingival recession. Materials and Methods: A cross-sectional analytical study involving 85 systemically healthy subjects aged 20–40 years was conducted. Gingival biotype was assessed by both probe transparency and transgingival probing methods. Probing depth and gingival recession were recorded for mandibular anterior teeth. SPSS software employed appropriate inferential statistics; p<0.05 was considered significant. Results: Thin gingival biotype was predominant, and there were statistically significant associations between biotype and both probing depth and gingival recession (p<0.05). Conclusion: Gingival biotype significantly influences periodontal parameters; therefore, routine assessment should be standard in periodontal diagnosis and treatment planning.