The best method for finding medicinally active ingredients in plant material is to screen for phytochemicals in the plants. The toxicity profile of a methanol extract of the stem bark of Andira inermis (Gwaska) and a phytochemical screening study were conducted in this study. Alkaloids, carbohydrates, cardiac glycosides, saponins, tannins, flavonoids, and steroids were all detected in the methanol extracts during the screening process, with the exception of tannins and flavonoids for lead acetate and Shinoda's test, which were not detected. The albino Wister rats' body weight did not significantly alter, and the LD50 was more than 5000 mg/kg, according to research using Lorke's method of toxicity profile in vivo. At 1500, 2500, and 5000 mg/kg doses of the extracts, respectively, the albino Wister rats also showed notable behavioural changes, including restlessness, erection of the hair coat, tiredness, and diarrhea. The findings showed that even at a high dosage of 5000 mg/kg, the Andira inermis methanol extract was not harmful to the experimental animals.