In this study, pumpkin seed oil extracted by the Soxhlet solvent method was converted into biodiesel using the transesterification process. This process involved the reaction between the extracted oil and alcohol (methanol) in the presence of a catalyst (NaOH) at an ideal temperature of 60 °C. The reaction resulted in the production of mono-alkyl esters (Biodiesel) and glycerol as byproducts. The functional groups of biodiesel were identified using the FTIR technique. The properties assessed included density, viscosity, color, flash point, cloud point, water content, pour point, total acid number, copper strip corrosion, and sulfur content. The properties were compared with the properties of fossil diesel according to ASTM D 6751 standard. Biodiesel was blended with fossil diesel (B20) as well as with fossil diesel and ethanol in different proportions. These blends were studied and compared with ASTM D 7467 standard. The results showed that biodiesel met all the requirements to be an alternative fuel. Moreover, its blends substantially complied with the standard.